OPTHALMOLOGY DISCOVERY SERVICES

YouTube

By loading the video, you agree to YouTube's privacy policy.
Learn more

Load video

As we envision the future, new therapies in ophthalmology rely on integrated approaches that cover the translational spectrum from “bench to bedside and back again”.

VectorB2B offers a plethora of integrated services in ophthalmology that combine extensive scientific expertise and state-of-the-art facilities every step of the way.

We offer a low risk “one stop shop” for large- or small-scale projects, made possible by the depth of experience and expertise of VectorB2B scientific team. VectorB2B will deliver high quality custom or standard drug discovery solutions on time and on budget.

ONE-STOP-SHOP IN Vision Science

VectorB2B offers the complete tool box of basic and translational research in vision sciences, including animal models for a variety of diseases and in vitro techniques to test drug candidates and provide insight into the molecular and cellular mechanisms underlying the pathology.

Our specialists have a broad experience in the establishment of in vitro & in vivo experimental models and possess a wide range of technical skills to evaluate treatments for optic neuropathies

photo-1486825586573-7131f7991bdd
photo-1572884267966-02340ebc90ac

The VectorB2B consortium harbors a wealth of expertise in neuroscience and neurological diseases that include Alzheimer’s or Parkinson´s Disease, autism, multiple sclerosis, amongst others. Our service portfolio ranges from research to clinical development:

IN VITRO MODELS

Primary retinal cell cultures

  • Rat retinal mixed cultures
  • Rat retinal ganglion cell cultures
  • Mouse retinal Müller cell cultures
  • Rat retinal microglial cell cultures
  • Bovine retinal endothelial cell cultures
  • Porcine retinal pigment epithelial cell cultures

 

Tissue culture

  • Organotypic retinal cultures from mouse, rat and human

 

Cell lines

  • Endothelial cells (HUVEC, human)
  • Retinal Müller cells (MIO-M1, human)
  • Microglial cells (BV-2, murine)

 

In vitro injury models

  • Elevated glucose concentration
  • Elevated Hydrostatic Pressure (EHP)
  • Excitotoxicity with N-methyl-d-aspartate (NMDA) or glutamate
  • Exposure to lipopolysaccharide (LPS) (inflammatory model)
  • Endothelial cells exposed to cytokines (breakdown of blood retinal barrier model)

IN VITRO ASSAYS

  • Cell viability assessment
  • Reactive oxygen species (ROS)
  • Production of nitric oxide (NO)
  • Cell proliferation assay
  • Cell migration assays
  • Phagocytosis assay
  • Cell death assays
  • Angiogenesis assays
  • Permeability assays
  • Leukocyte adhesion to the endothelium assay
  • Single cell intracellular calcium imaging
national-cancer-institute-9k4Fglw6eFQ-unsplash

What in vitro models do you need?

IN VIVO MODELS

ANIMAL MODELS OF DISEASE

Diabetic retinopathy

  • Rat model of prediabetes (administration of high concentration of sucrose in water)
  • Mouse and rat model of type 1 diabetes by intraperitoneal injection of streptozotocin
  • Mouse model of type I diabetes (Ins2Akita)

 

Glaucoma

  • DBA/2J mouse

 

Models of retinal injury

  • Transient retinal ischemia-reperfusion (I-R)
  • Excitotoxicity-induced retinal degeneration model (intravitreal injection of excitotoxic drugs, such as glutamate)
  • Ocular inflammation model (injection of LPS)
photo-1582560475093-ba66accbc424
louis-reed-pwcKF7L4-no-unsplash

IN VIVO MODELS

IN VIVO IMAGING

  • Fluorescein angiography
  • Optical coherence tomography (OCT)

 

FUNCTIONAL EVALUATION

  • Electroretinography (ERG)
  • Pattern electroretinography (pERG)
  • Scotopic threshold response (STR)
  • Intraocular pressure (IOP) measurement (Tonolab)
  • Optomotor response (OMR)

What in vivo models do you need?